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Pig MMP9 protein, His tag (active)

Pig MMP9 protein, His tag (active). GTX00050-pro
Pig MMP9 protein, His tag (active). GTX00050-pro
Pig MMP9 protein, His tag (active). GTX00050-pro

Cat. No. GTX00050-pro

Application

 Functional Assay

Species

 Pig
Package
10 μg ($319)
See all MMP9 protein products

APPLICATION

Application Note

MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant pig MMP9 (1000 ng, 500 ng, 100 ng, 10 ng, 1ng, 0.1 ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate- polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1 mg/ml) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In the experiment, using a heat-denatured MMP9 protein was as a negative control, and blood sample was as a positive control.

Observed MW

30 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ile169~Glu387

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

matrix metallopeptidase 9

Database

Research Area

DATA IMAGES

Pig MMP9 protein, His tag (active). GTX00050-pro

GTX00050-pro Functional Assay Image

Gelatin hydrolysis by various concentrations of GTX00050-pro Pig MMP9 protein (active).
Positive control: Blood sample
Negative control: Heat-denatured MMP9 protein

Pig MMP9 protein, His tag (active). GTX00050-pro

GTX00050-pro Image

WB analysis of GTX00050-pro Pig MMP9 protein (active).

Pig MMP9 protein, His tag (active). GTX00050-pro

GTX00050-pro Image

SDS-PAGE analysis of GTX00050-pro Pig MMP9 protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 319